1. The inhibition of diamine oxidase has been studied by using the following copper-chelating reagents: 1,10-phenanthroline; 2,2′-bipyridyl; 8-hydroxyquinoline (oxine); diethyldithiocarbamate and dithio-oxamide (rubeanic acid). 2. Addition of chelating reagent caused a rapid inhibition of enzyme to a degree dependent solely on the final inhibitor concentration. Addition of substrate gave linear initial rates of reaction showing that under these conditions the inhibition was not being rapidly reversed. 3. The inhibition has been investigated by using new graphical methods and has been found in all cases to involve the chelating agents completely removing two Cu2+ ions from the enzyme. An alternative possibility, involving ligand substitution, was eliminated. 4. A value of K=8.0×10−33m−2 has been found for the enzyme in equilibrium with 2 Cu2+ ions (i.e. β2, the stability constant for diamine oxidase/two Cu2+, is 32.1).
展开▼
